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News

  • Introduction to Targeted Antimicrobial Peptides

    Introduction to targeted antimicrobal peptides

    Natural antimicrobial peptides and substances, and their mechanisms of operations in host defenses, are things that the research world has been exploring since the late nineteenth century. In the early 1960s, the discovery of in vitro antibacterial actions of leukocyte extracts came into light, and it was majorly associated with the actions of basic proteins. Since then, there has been a lot of discovery and identification of a variety of cationic peptides that possess antimicrobial properties in tissues and other host cells present in nearly all the living species.
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  • Peptide Synthesis Services

    Peptides have a wide range of applications in science today. They are used in drugs and vaccines, to map enzyme binding sites, in the preparation of epitope-specific antibodies and to come up with a variety of novel enzymes among others. Back in the day, the process of peptide synthesis was not only labor-intensive but also resulted in very low peptide yields. Over the years, a lot has changed and the process has become much more efficient, leading to faster synthesis processes and higher yields.
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  • Freeze Drying Services – Everything You Need To Know

    Freeze drying is also known as cryodesiccation or lyophilization. It is a dehydration process with a variety of applications in the preservation of perishable products, and vital biological substances and assays needed to be stored for a long time. The process works by reducing the pressure in the substance so that frozen water can evaporate from solid to gas in a method known as sublimation. The first use of freeze drying was during the Second World War when it was used to preserve blood serum. Since then, technology has advanced and there have been a myriad of applications in multiple industries. Currently, the process is being used in industries such as pharmaceuticals, food processing, laboratory and testing, preservation of nutraceuticals, and preservation of dietary nutritional supplements among others.
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  • Lyophilization Services – Everything You Need To Know About the Process

    Lyophilization refers to the process of removing water molecules from a product after it is frozen and kept in a vacuum. The process is also known as freeze-drying, and is usually a long and complicated process that comes with a plethora of opportunities for potential applications. Peptides are an area of application of lyophilization, since there are a variety of pharmaceutical products that are usually subjected to the process. Apart from peptides, some of the usual targets for lyophilization include chemical API, collagen, proteins, enzymes, and oligonucleotide among others.
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  • Safety Above All During COVID-19

    We recognize the trust you place in us when ordering peptides and supplies for your research projects.

    That’s why we want to share an update on the actions we are taking at CanadaPeptide to keep you – and our team members – safe amidst the COVID-19 outbreak.

    “Safety Above All” is something we take incredibly seriously at CanadaPeptide. We are listening to recommendations from the World Health Organization (WHO) and other leading health organizations as we strive to help protect our customers and our team members.

    We are giving our team members facts about COVID-19 and reminding them to follow simple, but effective steps to keep them safe such as frequent handwashing and increased scrutiny over laboratory procedures.

    In light of the COVID-19 outbreak, you may have wondered, “Is that package or shipment safe to touch?” The WHO has answered that question for us. They’ve made it clear that it is safe to handle a package, even if it comes from a place where the virus has been reported.

    Please visit the WHO website for further details.

    As with prior disaster-related situations around the globe, we quickly opened our staff supplies to the public which include disposable medical gloves. We are all so proud to be part of a team that is always ready to extend a helping hand to communities in need.

    We recognize and appreciate the critical role we play research projects around the globe. We are currently implementing our contingency plans designed to help us continue to operate and limit any impact to your business and time sensitive research – all while keeping safety at the forefront.

    With new restrictions and limitations coming out daily to help contain the spread of the virus, we will be posting to our blog page the latest information on how we’re responding to this very dynamic situation.

    Thank you for putting your trust in us, and we remain focused on serving you.

  • How to Properly Interpret a QC Report

    Each and every custom synthesis project we work on will include a detailed QC report which is composed of an HPLC and MS analysis.

    We have created this guide to assist you in understanding the details contained in each analytical report document.

    This will demonstrate the the quality analysis that was performed for the peptide.

    Part 1 - The HPLC Analysis Report

    Peptide purification is sometimes a long and repetitive process. In most cases multiple purification steps are performed in order to purify and refine the peptide to the desired purity level required for your analytical procedure. Even after repetitive purification steps there will most always remain impurities in the final peptide product, as with most peptides 100% purity is not possible.

    Canada Peptide uses RP-HPLC (Reversed Phase High Performance Liquid Chromatography) methods to analyze the peptide purity.

    What is Reverse Phase High Performance Liquid Chromatography?

    HPLC is a widely used piece of equipment used in analysis. It functions on the principle of efficiently separating the components in both simple and highly complex mixtures. The substance to be analyzed is usually dissolved in water, this is what we call the analyte mixture. In many analyses this solution will also be mixed with an organic solvent or a specific acid in order to obtain a full dissolution of the compound before it is analysed.

    This analyte mixture is now ready for analysis by RP-HPLC and will be carried through the equipment by a mobile phase which is made up of water and another solvent or organic modifying solution. This new mixture will then be transported by pumps through an HPLC Column which contains the stationary phase. The stationary phase in most columns consists of an amount of small diameter particles made up of carbon chains with a specified surface length.

    As the mixture is pumped through the stationary phase column, the peptide and small impurities (known as the analytes) adsorb to the hydrophobic surface of the column / stationary phase.

    The amount of organic modifier or solvent mixture is slowly increased and measured, as this happens the analytes desorb into the mobile phase. Desorption of specific analytes are based on its natural properties, which translates into the retention time. These natural properties will remain in the column only for a certain amount of time.

    Many peptide bonds absorb UV light at their maximum capacity when the wavelength of 220 nanometers, therefore with many analyses it is useful to include a UV spectrometer to assist in detecting a peptide as it elutes from the stationary phase / column. This detector will provide a signal which is then converted to a graph (chromatogram) which plots the UV absorbtion and time it took to elute.

    The high resolution of eluted peptides and the gradient of elution predominantly depend on the columns and organic modifier or solvent that are chosen. Many different gradients are created in order to obtain a full separation for various peptides so that a high resolution is obtained for each peptide.

    Canada Peptide's team has a strong focus on creating HPLC protocols in order to achieve the most effective separation of the analyte and determine accurately the purity of each peptide.

    (1) HPLC Mobile Phase & Elution Gradient

    (2) Absoprtion Wavelength

    (3) Column Size and Type

    (4) Dead Zone

    (5) Elution Peak of the major components 

    (6) Peak Table

     

  • UHP Nitrogen

    New cylinder of Ultra High Purity Nitrogen UHP 5.0 installed and ready for use with the Liberty Blue Microwave Synthesizer.

    We also use the same grade of nitrogen for mass spectrometers.

  • Micromass OQ

    To follow up on the OQ posts, the team was doing the housekeeping and minor repairs on one of the HPLC/MS systems that are routinely used in analysis.

  • OQ on the Liberty Blue

    The team was performing routine OQ on one of the microwave peptide synthesizers as a part of the SOP.

    We were lucky enough to have the chance to grab some photos of the instrumentation laboratory and will be making regular posts as the team qualifies each equipment.

  • CUPW continues strikes in Winnipeg, Montreal and several other locations

    While negotiations continue, the Canadian Union of Postal Workers (CUPW) began strikes Thursday morning at the Winnipeg Mail Processing Plant and in Amherstburg, Strathroy, Sarnia and Milton, Ontario. Last night, strikes had also started in the greater Montreal region, Quebec and Saint John, New Brunswick. Strikes continue in St. John’s, Newfoundland.

    Montreal and Winnipeg key processing hubs

    Canada Post has been working hard to maintain service to Canadians, but Montreal and Winnipeg are key processing hubs for mail and parcels in Canada. The union’s rotating strikes will therefore have a significant impact on our operations. Canada Post will make every effort to minimize the impact, but customers across the country will continue to see delays for parcel and mail delivery.

    We thank our customers for their continued patience and apologize for the inconvenience this is causing.

     

    Full Details Here

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